MDA-MB-175-VII (ATCC® HTB-25)

Organism: Homo sapiens, human  /  Cell Type: epithelial  /  Tissue: mammary gland; breast/duct; derived from metastatic site: pleural effusion  /  Disease: ductal carcinoma

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Organism Homo sapiens, human
Tissue mammary gland; breast/duct; derived from metastatic site: pleural effusion
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties loosely adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease ductal carcinoma
Age 56 years
Gender female
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
Karyotype model number = 84; range = 82 to 89. The cell line is aneuploid female (XX/XXX/XXXX), with chromosome counts in the hypertetraploid to near-tetraploid range. All normal chromosomes are represented by at least one copy, with most chromosomes having three or four copies present per karyotype. Five marker chromosomes are found: del(1)p35), der(7)t(1;7)(q11;q32), unknown, t(2q;?), unknown isochromosome. Several are present in multiple copy. Alterations in the q arm of chromosome N1 are in accordance with the report by M.J. Siciliano, et al., Cancer Res. 39: 919, 1979.
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Derivation
This is one of a series of breast tumor lines (ATCC HTB-23 through HTB-26) isolated from pleural effusions and characterized by R. Cailleau and associates in 1973.
Clinical Data
56 years
Black
female
Tumorigenic Yes
Effects
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 100%
STR Profile
Amelogenin: X
CSF1PO: 7,8
D13S317: 11,12
D16S539: 11,13
D5S818: 11
D7S820: 11,12
THO1: 7
TPOX: 6,8
vWA: 15,16
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
PGM1, 2
PGM3, 1-2
Name of Depositor R Cailleau
References

Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

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References

Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247

Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202