Primary Mammary Epithelial Cells; Normal, Human (ATCC® PCS-600-010)

Organism: Homo sapiens, human  /  Tissue: Breast  / 

Permits and Restrictions

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Organism Homo sapiens, human
Tissue Breast
Morphology Cuboidal with a characteristic pattern of swirled cells; refractile
Growth Properties Adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Normal
Age Batch-specific
Gender Female
Ethnicity Batch-specific
Applications ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.
Product Format frozen 1 mL
Shipping Information frozen
Storage Conditions -130°C or below
Comments A complete solution to propagate mammary epithelial cells with excellent morphology and proliferation in serum-free conditions.
Complete Growth Medium
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the Mammary Epithelial Cell Growth Kit (PCS-600-040) just prior to adding them to the basal medium.
  3. Obtain one bottle of Mammary Epithelial Cell Basal Medium (PCS-600-030, 485 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each transfer.

  6. Component

    Volume

    Final Concentration

    rH-Insulin