PI3K Genetic Alteration Cell Panel

TCP-1028 ^™

The Phosphoinositide 3‑kinases (PI3Ks) family of enzymes is integral to several signaling pathways that mediate cell proliferation, survival, migration and vesicular trafficking. Member of the PI3K family are frequently mutated or altered in human cancers. For example, gain-of-function mutations in p110α (encoded by the PI3KCA gene) are observed in a variety of common cancers; and, these mutations are exclusively clustered in one of two hotspot regions, (i.e., exon 9 and 20), which correspond to either the helical or kinase domains of the protein (e.g., E545K and H1047R).

The PI3K Genetic Alteration Cell Panel (ATCC TCP-1028) is composed of ten human tumor cell lines from common cancer types that carry hotspot mutations within the PIK3CA gene. The PI3KCA status of each cell line has been sequenced and validated by ATCC. This panel is useful for PI3K pathway research and for anti-cancer drug discovery.

Components: ATCC CCL-225 HCT-15
ATCC CCL-237 SW948
ATCC CRL-1739 AGS
ATCC CRL-2577 RKO
ATCC HTB-112 HEC-1-A
ATCC HTB-121 BT-483
ATCC HTB-131 MDA-MB-453
ATCC HTB-178 NCI-H596
ATCC HTB-19 BT-20
ATCC HTB-27 MDA-MB-361
Product format: Frozen
Storage conditions: Vapor phase of liquid nitrogen

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Documentation

Product sheet

Instructions for use

BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Cells contain parainfluenza virus type 5 (HPIV-5)

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

General

Specific applications: This panel is useful for PI3K pathway research and for anti-cancer drug discovery.

Characteristics

Comments: The Phosphoinositide 3‑kinases (PI3Ks) family of enzymes is integral to several signaling pathways that mediate cell proliferation, survival, migration and vesicular trafficking. Member of the PI3K family are frequently mutated or altered in human cancers. For example, gain-of-function mutations in p110α (encoded by the PI3KCA gene) are observed in a variety of common cancers; and, these mutations are exclusively clustered in one of two hotspot regions, (i.e., exon 9 and 20), which correspond to either the helical or kinase domains of the protein (e.g., E545K and H1047R).

The PI3K Genetic Alteration Cell Panel (ATCC TCP-1028) is composed of ten human tumor cell lines from common cancer types that carry hotspot mutations within the PIK3CA gene. The PI3KCA status of each cell line has been sequenced and validated by ATCC. This panel is useful for PI3K pathway research and for anti-cancer drug discovery.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

For-profit Research Use License from MD Anderson Cancer Center

For-profit Organizations: For every order of this item, you must work directly with the contributor, MD Anderson Cancer Center to (i) negotiate a research-use license, and/or (ii) have the contributor provide authorization to ATCC to ship this item under your existing license. We cannot ship this item until we receive communication directly from MD Anderson Cancer Center that we are authorized to ship each order.

We are providing the following contact information, but this information may change without notice:
M.D. Anderson Cancer Center
Office of Technology Commercialization
Email: [email protected]

Once ATCC has received authorization from MD Anderson Cancer Center, your order will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

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