S194/5.XXO-1 (ATCC® TIB-19)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Disease: plasmacytoma; myeloma

Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Strain BALB/c
Applications
This line was derived from an IgA secreting mineral oil induced BALB/c myeloma.
Both heavy and light chains are synthesized but not secreted.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This line was derived from an IgA secreting mineral oil induced BALB/c myeloma.
Antigen Expression
H-2d; PC.1
Genes Expressed
immunoglobulin; monoclonal antibody; not secreted,H-2d; PC.1
Cellular Products
immunoglobulin; monoclonal antibody; not secreted
Comments
This line was derived from an IgA secreting mineral oil induced BALB/c myeloma.
Both heavy and light chains are synthesized but not secreted.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgA (not secreted); kappa light chain (not secreted)
Name of Depositor R Hyman, P Ralph, S Sarkar
References

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881

Genuario RR, Perry RP. The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J. Biol. Chem. 271: 4388-4395, 1996. PubMed: 8626789

Basic Documentation
References

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881

Genuario RR, Perry RP. The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J. Biol. Chem. 271: 4388-4395, 1996. PubMed: 8626789