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Recommended medium for CCL-61
What is the recommended growth medium for ATCC® CCL-61 ™, CHO-K1 cells?

It is important that CHO-K1 cells have proline (a component of NEAA) in the medium.   We culture these cells in ATCC-formulated F-12K medium (ATCC® 30-2004 ™) supplemented with high quality fetal bovine serum that has not been heat-inactivated(ATCC® 30-2020 ™).  ATCC does not use antibiotics when culturing these cells however if antibiotics are required for short term cultures, a 1% penicillin/streptomycin solution may be used and will not harm the cells.

Date Created02/21/2014 11:52 AM
Date Updated02/21/2014 11:53 AM

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