FAQ Home

Find Answers

Recommended medium for CCL-61
What is the recommended growth medium for ATCC® CCL-61 ™, CHO-K1 cells?

It is important that CHO-K1 cells have proline (a component of NEAA) in the medium.   We culture these cells in ATCC-formulated F-12K medium (ATCC® 30-2004 ™) supplemented with high quality fetal bovine serum that has not been heat-inactivated(ATCC® 30-2020 ™).  ATCC does not use antibiotics when culturing these cells however if antibiotics are required for short term cultures, a 1% penicillin/streptomycin solution may be used and will not harm the cells.

Date Created02/21/2014 11:52 AM
Date Updated02/21/2014 11:53 AM

Most Popular Answers

  1. Huh7 cell line
  2. ATCC HUVEC lines
  3. U-373 MG (ATCC® HTB-17)
  4. Passage number vs. population doubling level (PDL)
  5. Converting TCID[50] to plaque forming units (PFU)