It is important that CHO-K1 cells have proline (a component of NEAA) in the medium. We culture these cells in ATCC-formulated F-12K medium (ATCC® 30-2004 ™) supplemented with high quality fetal bovine serum that has not been heat-inactivated(ATCC® 30-2020 ™). ATCC does not use antibiotics when culturing these cells however if antibiotics are required for short term cultures, a 1% penicillin/streptomycin solution may be used and will not harm the cells.
|Date Created||02/21/2014 11:52 AM
|Date Updated||02/21/2014 11:53 AM