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Thawing NFkB-TIME cells
How should I thaw the NFkB-TIME cells (ATCC® CRL-4049 ™)?

Thaw the vial by gentle agitation in a 37°C water bath for approximately 2 minutes.

Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by spraying with 70% ethanol.

Transfer the vial contents to a centrifuge tube containing 9.0 mL of complete culture medium and centrifuge the cell suspension at approximately 125 x g for 5 minutes.

Discard the supernatant and resuspend the cells in fresh growth medium.

Count the cells and seed it at 4 - 6 x 103 cells/cm2 in culture vessel.

Incubate the culture at 37°C in a suitable incubator with a 5% CO2/95% air atmosphere.

Date Created02/13/2014 12:37 PM
Date Updated02/13/2014 12:42 PM

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