Primary microvascular endothelial cells may grow for 15 population doublings (PD) until they become senescent and undergo growth arrest.
The hTERT-immortalized microvascular cell lines, for example TIME (ATCC® CRL-4025 ™) and TIME-GFP (ATCC® CRL-4045 ™), are able to grow for more than 25 PD without changes in endothelial cell characteristics such as expression of CD31 and the ability to uptake AcLDL. Immortalized microvascular cell lines do not undergo grow arrest at later passages.
|Date Created||11/19/2013 09:51 AM
|Date Updated||03/27/2014 08:41 PM