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TIME-GFP growth kit selection
I notice ATCC has two different growth kits for microvascular endothelial cells.  Which one should I use for the ATCC® CRL-4045 ™ TIME-GFP cells?

Your experimental design will dictate which Endothelial Cell Growth Kit should be used.

Use of the Endothelial Cell Growth Kit - VEGF (ATCC® PCS-100-041 ™) is recommended only when the presence of this growth factor will not interfere with the research being performed.  The VEGF growth kit typically supports a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone.

Use of the Endothelial Cell Growth Kit - BBE (ATCC® PCS-100-040 ™), on the other hand, containing Bovine Brain Extract (BBE), can be used to mimic a more "authentic" culture environment without the addition of recombinant growth factors.

Date Created11/19/2013 09:46 AM
Date Updated03/27/2014 08:38 PM

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