It is normal to observe moderate to heavy debris and numerous floating cells in cultures of CRL-1730. The floating cells are viable and should be retained by gentle centrifugation and added back to the adherent population. If the populations are separated the culture may become too dilute and growth can slow (or stop completely). The cells should be fed with fresh medium several times each week, always keeping the floating cells in the same vessel as the adherent. These cells can appear granular and the edges can be difficult to see.
We recommend that the endothelial cell growth supplement (ECGS) must be high quality ECGS prepared from bovine neural tissue (Sigma Catalog no. E-2759 or equivalent) because we have found that these cells do not proliferate well if ECGS derived from bovine pituitary is used. ECGS is a poorly defined supplement and may vary from lot to lot.
|Date Created||02/21/2014 11:17 AM
|Date Updated||02/21/2014 11:18 AM