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Antibiotics in NFkB-TIME culture medium
Should I use antibiotics in the culture medium for ATCC® CRL-4049 ™ NFkB-TIME cells? What are the recommended concentrations? 

The culture medium for ATCC® CRL-4049 ™, NFkB-TIME cells should be supplemented with both blasticidin and hygromycin to ensure continued expression of hTERT and the NanoLuc® luciferase reporter gene, respectively.  Add blasticidin to a final concentration of 12.5 µg/mL and hygromycin to a final concentration of 20 µg/mL.
Date Created02/13/2014 12:01 PM
Date Updated02/13/2014 12:13 PM

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