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Adipogenic medium exchange for hTERT-MSCs

How should I exchange the adipogenic medium in the MSC culture? 

It is very important that the cell monolayer is not exposed to air during the adipogenic differentiation process so that the lipid vescicles do not burst.  Therefore, do not exchange 100% of the adipogenic medium at one time.  Also, do not tilt the plate or aspirate the medium during medium exchange.  Either of these two actions will expose all or part of the cell monolayer to air.
Date Created02/13/2014 02:01 PM
Date Updated02/13/2014 02:05 PM

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