These cells adhere and grow as small connecting clusters of epithelial-like cells. Initially, some moderate to heavy suspension of cells and debris is present. As they grow more confluent, the adhered cell groups will increase in density and form a monolayer.
Under culture conditons used at the ATCC, when a T75 flask is initiated at a 1:15 dilution, the cells reach approximately 40% confluence after 6 days of incubation, during which time fresh media is added. After 6 days, the medis is changed completely and the cells are re-incubated at 37C. After an additional 4 days, the cells are apprxoimately 70-80% confluent and can be sub-cultured to three T75 flasks.
|Date Created||02/10/2014 12:46 PM
|Date Updated||02/10/2014 12:53 PM