Primary Cell Culture Reagents and Media

  • Fibroblast Basal Medium (ATCC® PCS-201-030)

    ATCC® Number: PCS-201-030

    Quantity: 480 ml
    Storage: 2 to 8°C; protect from light
    Applications:

    Fibroblast Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support fibroblasts derived from normal human epidermis. Fibroblast Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.

    1. To support the proliferation and plating efficiency of fibroblasts (e.g., Dermal Fibroblasts, Normal, Human Neonatal [ATCC PCS-201-010], Dermal Fibroblasts, Normal, Human Neonatal, Mitomycin C Treated [ATCC PCS-201-010]), Fibroblast Basal Medium must be supplemented with one of the following kits:
      1. Fibroblast Growth Kit–Serum-Free (ATCC PCS-201-040)
      2. Fibroblast Growth Kit–Low Serum (ATCC PCS-201-041)
    2. Optional media supplements:
      1. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
      2. Phenol Red (ATCC PCS-999-001)

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  • Mammary Epithelial Cell Basal Medium (ATCC® PCS-600-030)

    ATCC® Number: PCS-600-030

    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Mammary Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium designed to support epithelial cells derived from normal human female breast. Mammary Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of mammary epithelial cells ATCC PCS-600-010, Mammary Cell Basal Medium must be supplemented with the Mammary Epithelial Cell Growth Kit (ATCC PCS-600-040).

    ATCC’s Human Mammary Epithelial Cells cultured in Mammary Epithelial Cell complete medium (basal medium plus growth kit) is an optimal serum-free culture model for many research areas. Common uses of HMEC include the study of breast cancer development, three dimensional culture and carcinogen screening.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

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  • Airway Epithelial Cell Basal Medium (ATCC® PCS-300-030)

    ATCC® Number: PCS-300-030

    Quantity: 485 mL
    Storage: 4 to 8°C, protect from light
    Applications:

    Airway Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This serum-free system is designed to support airway epithelial cells derived from normal human lung. Airway Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of various types of airway epithelial cells, Airway Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit. When using this complete media system, the growth of airway epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. 

    1. For airway epithelial cells derived from bronchial/tracheal tissue (e.g., Primary Bronchial/Tracheal Epithelial Cells, Normal, Human, ATCC PCS-300-010), supplement Airway Epithelial Cell Basal Medium with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040).
    2. For small airway epithelial cells (e.g., Primary Small Airway Epithelial Cells, Normal, Human, ATCC PCS-301-010), supplement Airway Epithelial Cell Basal Medium with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040).
    3. Optional media supplements:
      1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
      2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
      3. Phenol Red (ATCC PCS-999-001)

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  • Renal Epithelial Cell Basal Medium (ATCC® PCS-400-030)

    ATCC® Number: PCS-400-030

    Quantity: 485 mL
    Storage: 2 to 8°C, protect from light
    Applications:

    Renal Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. Renal Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of renal epithelial cells (e.g., Primary Renal Proximal Tubule Epithelial Cells, Normal, Human, ATCC PCS-400-010); Primary Renal Cortical Epithelial Cells, Normal, Human, (ATCC PCS-400-011); Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC PCS-400-012), Renal Cell Basal Medium must be supplemented with the Renal Epithelial Cell Growth Kit (ATCC PCS-400-040).

    Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution  (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

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