pBLCAT2 (ATCC® 37527)

Applications: vector for other usesvector permitting construction of fusion proteins  /  Depositors: B Luckow, G Schutz

Designations pBLCAT2
Depositors B Luckow, G Schutz
Biosafety Level 1
Vector Information
Size (kb): 4.5000000000000000
Vector: pBLCAT2 (plasmid)
Promoters: Promoter HSV TK
Construction: pUC18, SV40, cml, herpes simplex virus TK promoter
Construct size (kb): 4.5
Features: marker(s): ampR
promoter: HSV TK
replicon: pMB1
vector for other uses
vector permitting construction of fusion proteins
Restriction digests of the clone give the following sizes (kb): BamHI--4.2; HindIII--4.2; BglII--4.2; ClaI--uncut; SmaI--4.3.
Plasmid to simplify construction of hybrid CAT coding sequences.
The ClaI site appears to have been destroyed during cloning.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Luckow B, Schutz G. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res. 15: 5490, 1987. PubMed: 3037497

Shipped freeze-dried
Shipping Information Distributed: freeze-dried