Authentication and Characterization of Animal Cell lines

Towards Best Practices in Cell Culture

2/27/2014


Over the years, numerous cell lines have been shown to be misidentified due in part to poor techniques, inadequate authentication protocols, and sharing of unauthenticated cell lines amongst researchers. Technological advances have given rise to improved capabilities. Cell line authentication and characterization now requires a comprehensive strategy that employs several complementary technologies for systematic testing for morphology, microbial contaminations, cellular cross-contamination, as well as functionality. An overview of the current technologies used to authenticate and characterize animal cell lines will be presented.

Presenters

Yvonne A. Reid, Ph.D.

Yvonne Reid, Ph.D.,
Consultant, Scientist

Yvonne A. Reid, Ph.D., is a Consultant and former employee at ATCC. Dr. Reid has been at the forefront of developing and standardizing methods to characterize cell lines by enzymology, karyotyping, and more recently DNA profiling, and to propagate, cryopreserve, and track validated batches of thousands of animal cell lines and strains. These validation protocols have been adopted by cell banks around the world. In addition, Dr. Reid has been heavily involved in developing guidelines for authenticating cell lines and cell types.