Best Practices in Cryopreservation

4/21/2016


Abstract: Cryopreservation is the use of very low temperatures to structurally preserve intact living cells and tissues. Normally, the freezing of water in cells causes catastrophic damage to cellular structure by physical damage of ice formation and increased imbalance of solutes. Cryopreserving cells with the proper cryoprotectants and techniques will maximize viability of cells for cell culture. This webinar presentation will discuss best practices for cryopreservation focusing on determining optimal freezing rates and cryoprotectants, selecting proper containment units, managing a biorepository, and handling cells post-thaw. Emphasis will be placed on reviewing time-proven techniques while introducing newer innovative approaches to maximize reliability in cryopreservation for modern day cell culture.

Key Points:

  • For the most accurate administration of DMSO, use Serum Free Cell Freezing Media already formulated with exactly 10% DMSO
  • A controlled cooling rate (1 – 3°C /min) is necessary to maximize cell viability when freezing down cell lines
  • The CoolCell® is a reliable and consistent cryo-container

Presenters

Steve Budd

Steven Budd, M.S., M.B.A.,
Product Line Business Specialist, ATCC

Steven Budd is a Product Line Business Specialist that manages the cell culture reagents at ATCC. He has 4 years of experience in the product management of scientific tools. Before that, he gained 4 years of experience in biomedical research and cell culture as a research specialist at the University of North Carolina at Chapel Hill. Mr. Budd has a M.S. in Biology from the University of North Carolina at Wilmington and an M.B.A. from North Carolina State University.