Excellence in Research webinar series

Your trusted resource for the world’s largest and most diverse collection of human and animal cell lines, microorganisms, biological products, standards, and custom services announces the 2016 Excellence in Research webinar series. The series will showcase the implementation of leading technologies,  the importance of standards, and the application of new ATCC products and  services to advance your scientific research. The webinars will broadcast live for 30 to 45 minutes and will be followed by a 10 minute question and answer  period. Register to join the webinar that best meets your schedule.


Steven Budd Keeping Cells Happy – Topics in Cell Health Maintenance and Viability

Steven Budd, M.S., M.B.A., Product Line Business Specialist, ATCC
September 29, 2016

Abstract: Good practices in cell heath are vital to safeguarding the success of long-term cell cultures and obtaining reproducible experimental results. Cell health covers a wide range of topics, from ensuring that your cells are thriving in culture to having confidence that they are characterized correctly. This webinar will explore topics such as cell viability assays that measure growth, authentication of cell lines via short tandem repeat (STR) profiling and mycoplasma detection, as well as aseptic technique and cryopreservation. From services to techniques, we will discuss ATCC’s role in ensuring the health and maintenance of cells in culture.

Key Points:

  • Mycoplasma infection can chronically affect the well-being of cells in culture without being easily detected by visual observation. Specialty kits or routine screening are required to determine if cell cultures are infected.
  • STR profiling detects misidentification or cross-contamination of cell lines, which can invalidate research efforts.
  • When culturing specialty cells, such as stem cells or primary cells, certain considerations regarding the choice of media and reagents must be taken.

Biography:

Steven Budd is a Product Line Business Specialist that manages the cell culture reagents at ATCC. He has 4 years of experience in the product management of scientific tools. Before that, he gained 4 years of experience in biomedical research and cell culture as a research specialist at the University of North Carolina at Chapel Hill. Mr. Budd has a M.S. in Biology from the University of North Carolina at Wilmington and an M.B.A. from North Carolina State University.

Register for this session 12:00 PM ET.

The Biology of Anaerobic Bacteria and Predominant Propagation Practices

Allison Faust, Senior Biologist, ATCC
and 
Nancy Krueger, Senior Biologist, ATCC
November 3, 2016

Abstract: Culturing anaerobes in the laboratory can be challenging. Due to the copious growth requirements and the plethora of anaerobic organisms, knowing the optimal conditions for your anaerobic organism is essential. In this webinar, we will present the various methods to achieve successful growth conditions for a wide variety of anaerobes. We will consider topics such as common anaerobic gas mixtures, media selection, and obtaining anaerobic conditions in the lab. Taking into account individual laboratory constraints, we will also discuss several propagation methods available today. Using specific examples from the ATCC collection, we will demonstrate these techniques.

Key Points:

  • There are numerous types of anaerobic bacteria with various atmospheric and nutritional requirements from clinical gut and wound isolates to environmental extremophiles. 
  • Creating and maintaining anaerobic atmospheric and nutritional conditions are critical for the successful growth of anaerobic bacteria. 
  • The propagation of anaerobic bacteria has evolved throughout the years.  The discovery of new isolates has brought about the need for more robust culturing technology. 

Biographies:

Allison Faust is a Senior Biologist in the Laboratory Testing Services laboratory at ATCC. She has a B.S. in Biology and extensive experience in culturing and quality control testing the vast array of bacterial and protist isolates offered at ATCC. She has participated in anaerobic workshops and internal anaerobic studies to optimize the anaerobes offered at ATCC. 

Nancy Krueger is a Senior Biologist in the Bacteriology and Mycology departments at ATCC. During the last 8 years, she has performed quality control and propagation of a variety of bacteria, fungi, and yeast. She has also participated in an internal study to determine the ideal propagation and test methods for anaerobes. Ms. Krueger has a B.S. in Biology from George Mason University.

Register for this session 12:00 PM ET.

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