Application Notes

This study showcases the development of a NIH/3T3 clonal derivative that maintains the original contact inhibition characteristics associated with this cell line.

Our newly developed animal by-product free McCoy’s 5A medium was tested across several ATCC cell lines for performance and was found to be a suitable substitute for the current McCoy’s 5A Medium.

This study demonstrates the use of three robust, responsive, and reproducible GAS-Luc2 reporter solid tumor cell lines for the assessment of T cell and other immune/tumor microenvironmental cell-mediated immune responses triggered by PD-1/PD-L1, CD-155/TIGIT, or B7-H3 (CD276) immune checkpoints.

In this application note, we demonstrate the application of our high-quality quantitative gDNA control material in validating quantitative PCR (qPCR) assays designed to detect residual host gDNA impurities in biologics.

In this application note, we demonstrate the application of our high-quality quantitative gDNA control material in validating quantitative PCR (qPCR) assays designed to detect residual host gDNA impurities in biologics.

In this application note, we demonstrate the application of our high-quality quantitative gDNA control material in validating quantitative PCR (qPCR) assays designed to detect residual host gDNA impurities in biologics.

In this application note, we demonstrate the application of our high-quality quantitative gDNA control material in validating quantitative PCR (qPCR) assays designed to detect residual host gDNA impurities in biologics.

In this application note, we demonstrate the application of our high-quality quantitative gDNA control material in validating quantitative PCR (qPCR) assays designed to detect residual host gDNA impurities in biologics.

In this application note, we demonstrate the application of our high-quality quantitative gDNA control material in validating quantitative PCR (qPCR) assays designed to detect residual host gDNA impurities in biologics.

This study determines if 3T3-L1 adipocyte differentiation is dependent on contact inhibition and tests the OP9 cell line as a possible substitute for 3T3-L1 cells in adipocyte assays.

This study demonstrates the use of ATCC Minis as quality control strains for microbial identification on the VITEK 2 platform.

A flow cytometry-based assay using K-562 cells stably expressing green fluorescent protein to quantify the cytotoxic activity of primary human natural killer cells.

This study will test the ability of primary human bronchial/tracheal epithelial cells (HBECs) to differentiate into mature airway tissue using an air-liquid interface (ALI) culture model.

This study demonstrates keto-reductase enzyme activity amongst a group of 26 ATCC strains and the potential use of this activity in industrial applications aimed at outperforming traditional chemical-based approaches.

In this application note, we show that self-constructed airway models may serve as useful tools future airway toxicity research.

This study examines the ability of a novel medium to stimulate neural progenitor cells to differentiate into multiple types of neurons.

ATCC has developed 4 quantitated synthetic molecular standards for Dengue virus (DENV) serotypes 1-4 for use as positive controls in qRT-PCR assays. Read this study to explore the development and application of these molecular standards.

In this study, ATCC designed and developed a quantitative synthetic molecular standard for Monkeypox virus to serve as a safe and reliable positive control material when conducting diagnostics assays.

Three human iPSC lines were tested for their ability to generate gastrointestinal organoids using defined culture media in a 2-D/3-D culture system using ATCC Cell Basement Membrane.

This report describes a straightforward and robust method of generating neurospheres from neural progenitor cells

An isogenic cell line was created to model cancer patients with the echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) fusion oncogene, a key oncogenic driver, and then tested for its sensitivity to known selective inhibitors of ALK.

In this study, we used CRISPR/Cas9 gene-editing technology to create cell lines capable of producing model clinical viruses and adeno-associated viruses at titers much higher than the parental cell lines, providing an efficient method for biopharmaceutical companies to increase production while reducing associated costs.

We created three different hTERT-immortalized human primary renal proximal tubule epithelial cells (RPTEC) that stably express the OAT1, OCT2, and OAT3 proteins. We then tested the ability of these cells to intracellularly convey known SLC protein substrates.

This study shows that an immortalized keratinocyte cell line, Ker-CT, is able to differentiate into organotypic skin equivalents in a 3-D culture model

This study will demonstrate the differentiation potential of cryopreserved primary human CD14+ monocytes towards macrophage and dendritic cell lineages for applications including cancer immunology and vaccine development,

As part of our initiative to enhance the authentication of our products, we identified the key challenges regarding existing microbial genome databases and developed a solution for improving the quality of reference genome sequences.

We have developed green fluorescent protein (GFP)-labled strains of various Gram-negative opportunistic pathogens from the ATCC collection. Here, we demonstrate the use of these labeled strains in studying bacterial pathogenesis, host-pathogen interactions, and compound screening assays.

Here, we describe a protocol for the initiation, culture, and subsequent cryopreservation of mouse small intestinal organoids starting from previously cryopreserved primary tissue–derived organoids.

Explore the development and application of a panel of A. fumigatus strains exhibiting various levels of sensitivity to common antifungal drugs.

Learn how ATCC-designed quantitative mycoplasma DNA controls can be used to evaluate the sensitivity of two different molecular-based detection systems.

This report showcases an optimal method of fabricating airway models consisting of human bronchial tracheal epithelial cells grown under air-liquid interface (ALI).

In this study, we evaluate the use of green fluorescent protein (GFP)-labeled strains representing several clinically relevant Shiga toxin-producing Escherichia coli (STEC) serogroups associated with foodborne disease as positive controls.

In this study, we demonstrate that ATCC NGS Standards combined with the One Codex data analysis module provide a comprehensive solution for standardizing data from a wide range of sources, and generating consensus among microbiome applications and analyses.

In this study, we demonstrate the utility of molecular standards for Human herpesvirus 1 and 2 in the generation of standard curves for the detection and quantification of viral load.

Explore the use of quantitative synthetic molecular standards for Norovirus genogroups I and II in assay development.

Explore the development and validation of quantitated synthetic molecular standards for Hepatitis B virus (HBV) and Hepatitis C virus (HCV) and their applications as positive controls in molecular-based assays.

This study will examine the differentiation and expansion potential of bone marrow-derived (BM) CD34+ cells into erythroid cells, megakaryocytes, and myeloid precursor cells in a liquid, non-adherent culture format.

In this study, we describe the development and quality control of the ATCC virome standards and present application data demonstrating their use throughout the different stages of a typical virome analysis workflow.

In this study, we created a HEK293T/17 cell line that stably expresses the human organic anion transporter (OAT1) and tested its transport capabilities.

This report describes ATCC luciferase reporter solid and liquid tumor cell lines that express tumor antigens such as CD19, CD20, and HER2. Learn how you can incorporate these target cells into your T-Cell cytotoxicity assays to enable your breakthroughs in immuno-oncology.

In this study, we demonstrate that TIME cells maintain their normal growth rate and endothelial character at late passage when grown in ATCC’s VCBM-VEGF medium, but not in the previously recommended Lonza EGM™-2 MV BulletKit medium.

Discover how to tailor your dissociation method to your cell line to ensure successful subculture.

To facilitate the rapid and reliable detection of Mycoplasma in cell culture, ATCC offers the PCR-based Universal Mycoplasma Detection Kit. Explore how to use the kit to check your cultures for mycoplasma contamination.

In this application note, we describe the construction and application of ATCC Spike-in Standards as a tool for quantitative metagenomic analysis.

This study will demonstrate the use of the ATCC Vibrio campbellii Panel as a non-pathogenic model for AI-2-based quorum sensing pathways.