OAT1 HEK 293T/17 (ATCC® CRL-11268G-1)

Organism: Homo sapiens, human  /  Tissue: kidney  /  Disease: normal

Permits and Restrictions

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Organism Homo sapiens, human
Tissue kidney
Product Format frozen 1.0 mL
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain Adeno and SV-40 viral DNA sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age fetus
Gender female
Applications Toxicity testing for OAT1 transporter
Storage Conditions liquid nitrogen vapor phase
Images Cell Micrograph of ATCC CRL-11268G-1
Receptor Expression OAT1>75% positive
Comments

Drug screening; toxicity testing for organic anion 1 transporter

Complete Growth Medium DMEM (ATCC 30-2002), 10% FBS (ATCC 30-2020), 3µg/mL Blasticidin (Life Technologies A11139-03)
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes.

  1. Remove and discard spent medium.
  2. Briefly rinse with dPBS (ATCC 30-2200), 1 mL per 25 cm2 and discard rinse solution.
  3. Add 0.25% trypsin  (ATCC 30-2101), 1mL per 25 cm2.  Place at 37°C for 2-3 minutes, until 90% of the cells have detached. 
  4. Rapt flask gently to ensure cells are detached. Add complete growth media, 1 mL per 25 cm2 to neutralize trypsin.
  5. Centrifuge cells at 200 x g for 5 min at room temperature.
  6. Remove supernatant. Resuspend pellet in 5 to 10 mL Complete Growth Medium.
  7. Count cells, and seed 5.0 x 103 to 8.0 x 103 viable cells/cm2 to new culture vessels.

Subculture every 2-3 days at 8000 cells/cm2

Cryopreservation

Serum Free Cell Freezing Media (ATCC® 30-2600)

Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Cells per Vial >1.0 x 106 cells/vial
Volume 1.0 mL
STR Profile Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13,14
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,18,19
Sterility Tests Bacteria and Yeast: Negative
Mycoplasma: Negative
Viral Testing Hepatitis B: Negative
Hepatitis C: Negative
HIV: Negative
Name of Depositor ATCC
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • For-profit customers must obtain a research use license agreement from the Contributor prior to shipment.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
FAQ's
  1. Growth of OAT1-HEK ATCC CRL-11268G-1 cells
    Yes.  These cells grow similar to CRL-11268 with a slight reduction in growth rate most likely due to the blasticidin in the media which maintains selective pressure.
    Date Updated: 5/17/2016
  2. Generation of OAT1-HEK ATCC CRL-11268G-1 cells
    The OAT1-HEK cells were generated by stably transfecting 293T/17 [HEK 293T/17] (ATCC® CRL-11268 ™) cells with cDNA for human organic anion transporter 1 (hOAT1) following selection with...
    Date Updated: 5/17/2016
  3. Expression of OAT1
    The ATCC
    Date Updated: 5/23/2016
Restrictions

This material’s use is governed by the MTA and the following restrictions: (1) Prior to purchase, for-profit entities must obtain a research use license from Rockefeller University and (2) when a Commercial Use is contemplated, for-profit and non-profit entities must obtain a Commercial Use license from Rockefeller University.  Rockefeller University will be informed of all customers that purchase this product.  For information on executing a license to use this product for research (for-profit entities) or Commercial Use (for-profit and non-profit entities), contact Rockefeller University, Office of Technology Transfer, 1230 York Avenue, New York, NY 10065 Attn: Nidhi Sabharwal, Assistant Director, Marketing & Licensing at nsabharwal@rockefeller.edu.