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Human respiratory syncytial virus, High titer (ATCC® VR-1580PQ)

Classification: Pneumoviridae, Orthopneumovirus, Human orthopneumovirus  /  Product Format: frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL

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Classification Pneumoviridae, Orthopneumovirus, Human orthopneumovirus
Deposited As Respiratory syncytial virus
Agent Human respiratory syncytial virus, High titer
Strain 18537
Applications Virucide testing
Respiratory research
In vitro infectivity assay
Antiviral studies
Spiking studies
ELISpot assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL
Storage Conditions -70°C or colder
Images VR-1580PQ images
Comments

ATCC® VR-1580PQ™ is a high-titer, live virus suspended in 50 mM Tris-HCl + 150 mM NaCl + 1 mM EDTA + dH2O. This product was prepared from ATCC® VR-1580™ via concentration through a sucrose cushion.

The early passage history of this item is unknown.
Effect on Host
CPE, cell rounding, sloughing, and syncytia
Recommended Host
Hep-2 cells (ATCC® CCL-23™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01 to 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 3-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, cell rounding, sloughing, and syncytia
Name of Depositor RM Chanock, National Institute of Allergy and Infectious Diseases; ATCC
Source Respiratory secretions from child with acute respiratory disease seen at Children’s Hospital of the District of Columbia, Washington, DC, 1962. Treated ATC VR-1401™ to removed Mycoplasma at ATCC.
References

Coates HV, et al. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am. J. Epidemiol. 83: 299-313, 1966. PubMed: 5933417

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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