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Human rhinovirus 1B, Purified (ATCC® VR-1645PQ)

Classification: Picornaviridae, Enterovirus, Human rhinovirus A  /  Product Format: frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL

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Classification Picornaviridae, Enterovirus, Human rhinovirus A
Agent Human rhinovirus 1B, Purified
Strain B632
Applications Respiratory research
In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL
Storage Conditions -70°C or colder
Genome Sequenced Strain

Human rhinovirus 1B, complete genome

Comments ATCC® VR-1645PQ™ is a high-titer, purified, live virus suspended in 0.01% BSA + 25 mM HEPES + 10 mM NaCl + DPBS. This product was prepared from ATCC® VR-1645™ via purification through sucrose cushion.

Strain B632 is the prototype strain for Human rhinovirus 1B
Effect on Host
CPE, rounding and sloughing
Recommended Host
H1HeLa (ATCC® CRL-1958™)
Growth Conditions

Temperature: 33°C

Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere rocking continuously. End adsorption by adding virus growth medium.

Incubation: 2-7 days

Effect on Host
CPE, rounding and sloughing
Name of Depositor National Institute of Allergy and Infectious Diseases; ATCC
Source Presumed from human throat washings. Treated ATCC® VR-1111™ to remove mycoplasma at ATCC®.
References

Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967.

Hughes PJ, et al. The nucleotide sequence of human rhinovirus 1B: molecular relationships within the rhinovirus genus. J. Gen. Virol. 69 (PT1): 49-58, 1988. PubMed: 2826669

Taylor-Robinson D, Tyrrell DA. Serotypes of viruses (Rhinoviruses) isolated from common colds. Lancet 1: 452-454, 1962. PubMed: 13920011

Cross References

Nucleotide (GenBank) : D00239.1 Human rhinovirus 1B, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation