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Enterovirus D68, High titer (ATCC® VR-1823PQ)

Product Format: frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL

Permits and Restrictions

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Agent Enterovirus D68, High titer
Strain US/MO/14-18947
Applications Virucide testing
Respiratory research
In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL
Storage Conditions -70°C or colder
Images VR-1823PQ images
Comments ATCC® VR-1823PQ™ is a high-titer, live virus suspended in 50 mM Tris-HCl + 150 mM NaCl + 1 mM EDTA + dH2O. This product was prepared from ATCC® VR-1823™ via concentration through a sucrose cushion.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
RD cells (ATCC® CCL-136™)
Growth Conditions

Temperature: 33°C

Recommendations for Infection: Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere rocking continuously at 0.5 rpm. End adsorption by adding virus growth medium.

Incubation: 2-3 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor Centers for Disease Control and Prevention
Source Nasopharyngeal swab from patient with respiratory illness, Missouri, September 2014
Year of Origin 2014
References

Brown BA, et al. Seven Strains of Enterovirus D68 Detected in the United States during the 2014 Severe Respiratory Disease Outbreak. Genome Announc 2(6): e01201-14, 2014. PubMed: 25414503

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
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