Zika virus, Heat Inactivated (ATCC® VR-1843HK)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen 0.25 mL per vial

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Classification Flaviviridae, Flavivirus
Deposited As Zika virus
Agent Zika virus, Heat Inactivated
Strain PRVABC59
Applications This product is intended for use as a positive control in molecular assays for the qualitative detection and identification of ZIKV. See the Certificate of Analysis for details. ATCC VR-1843HK has not been tested in other applications. It does not contain intact ZIKV genomic RNA, and should not be used as a source of nucleic acid for transfection or for RT-PCR of “gene-length” amplicons.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.25 mL per vial
Storage Conditions -70°C or colder
Genome Sequenced Strain

Zika virus strain PRVABC59, complete genome

Comments This product is a preparation of Zika virus (ZIKV) PRVABC59 that has been inactivated by heating to 65°C for 30 minutes.

Each vial contains approximately 0.25 mL of heat-inactivated, clarified cell lysate and supernatant from Vero E6 cells infected with ZIKV PRVABC59. The vial should be centrifuged prior to opening.

The long-term stability of this preparation is not known at this time. It is recommended that users confirm the activity of the product if not used within three months of receipt.
Source Human serum specimen, Puerto Rico, December 2015
References

Lanciotti RS, et al. Phylogeny of Zika Virus in Western Hemisphere, 2015. Emerg Infect Dis 22: 933-935, 2016. PubMed: 27088323

Cross References

Nucleotide (GenBank) : KU501215 Zika virus strain PRVABC59, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation