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Zika virus, High titer (ATCC® VR-1843PQ)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL

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Classification Flaviviridae, Flavivirus
Deposited As Zika
Agent Zika virus, High titer
Strain PRVABC59
Applications Vector-borne disease research
In vitro infectivity assay
Antiviral studies
Spiking studies
ELISpot assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL
Storage Conditions -70°C or colder
Genome Sequenced Strain

Zika virus strain PRVABC59, complete genome

Images VR-1843PQ images
Comments ATCC® VR-1843PQ™ is a high-titer, live virus suspended in culture medium. This product was prepared from ATCC® VR-1843™ via concentration by centrifugal filtration.
Effect on Host CPE, refractile rounding and sloughing
Recommended Host
Vero C1008 cells (ATCC® CRL-1586™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 60-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host CPE, refractile rounding and sloughing
Name of Depositor B Russell, Centers for Disease Control and Prevention
Source Human serum specimen, Puerto Rico, December 2015
Year of Origin 2015
References

Dick GW, Kitchen SF, and Haddow AJ. Zika Virus. I. Isolations and Serological Specificity. Trans R Soc Trop Med Hyg 46: 509-520, 1952. PubMed: 12995440

Zhu Z, et al. Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic. Emerg Microbes Infect 5: e22, 2016.

Cross References

Nucleotide (GenBank) : KU501215 Zika virus strain PRVABC59, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation