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Zika virus (ATCC® VR-1848)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen 1.0 mL per vial

Permits and Restrictions

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Classification Flaviviridae, Flavivirus
Agent Zika virus
Strain R103451
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Genome Sequenced Strain

Zika virus isolate 103451, complete genome

Images VR-1848 images
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
Vero cells (ATCC® CCL-81™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1 hour at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 4-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed throughout monolayer.

Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor BJ Russell, Centers for Disease Control and Prevention
Source Placenta of a human isolated on January 6, 2016 infected from travel to Honduras in 2015
Cross References

Nucleotide (GenBank) : KX262887 Zika virus isolate 103451, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
FAQs
  1. Is Zika a new virus
    No. According to the CDC, Zika previously has been reported in tropical Africa, Southeast Asia, and the Pacific Islands. In May 2015, the Pan American Health Organization (PAHO) issued an alert r...
    Date Updated: 2/5/2016
  2. What is Zika virus
    Zika virus is a single-stranded RNA virus of the Flaviviridae family, genus Flavivirus (which also includes the Dengue, and West Nile Viruses). Zika virus is transmitted to humans primarily ...

    Date Updated: 3/3/2016
  3. Does ATCC distribute the Zika virus
    Yes, Zika virus (ATCC® VR-84™) strain MR766 has been distributed by ATCC since 1953.  The virus is available through ATCC, and our designated MOI (Multiplicity of Infection)
    MOI stands for "Multiplicity of Infection." It is the ratio of infectious virus particles to the number of cells being infected. The recommended MOI is in the range of 0.1 (meaning that you inocu...
    Date Updated: 3/13/2014