Antibody Class: IgG2ck
Mouse monoclonal antibody prepared against the envelope (E) glycoprotein of Zika virus (ZIKV) was purified from ascites fluid by protein G affinity chromatography. Ascites formation was induced by injecting approximately 2.5 x 106 cultured hybridoma clone ZV-16 cells into the peritoneal cavities of pristine-primed mice. The B cell hybridoma was generated by the fusion of P3X6 Ag 126.96.36.199 mouse myeloma cells with immunized mouse splenocytes. The ZV-16 antibody specifically recognizes the E glycoprotein, is non-neutralizing, and does not cross-react with Dengue, Japanese encephalitis, or West Nile viruses.
ATCC® VR-1866™ specifically recognizes ZIKV-infected cells in indirect immunofluorescence assays. The clone ZV-16 antibody is also reported to function in ELISA and in western blot assays performed under non-reducing conditions.