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Monoclonal Anti-Zika virus envelope protein, clone ZV-16 (Immunoglobin G Mouse) (ATCC® VR-1866)

Product Format: frozen
Each vial of ATCC® VR-1866™ contains approximately 100 µL of purified monoclonal antibody in PBS, pH 7.4. The concentration, expressed as mg per mL, is shown on the Certificate of Analysis for each lot.

Permits and Restrictions

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Agent Monoclonal Anti-Zika virus envelope protein, clone ZV-16 (Immunoglobin G Mouse)
Applications Emerging infectious disease research
Vector-borne disease research
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Each vial of ATCC® VR-1866™ contains approximately 100 µL of purified monoclonal antibody in PBS, pH 7.4. The concentration, expressed as mg per mL, is shown on the Certificate of Analysis for each lot.
Storage Conditions -20°C or colder. Repeated freeze-thaw cycles should be avoided.
Comments

Antibody Class: IgG2ck

Mouse monoclonal antibody prepared against the envelope (E) glycoprotein of Zika virus (ZIKV) was purified from ascites fluid by protein G affinity chromatography. Ascites formation was induced by injecting approximately 2.5 x 106 cultured hybridoma clone ZV-16 cells into the peritoneal cavities of pristine-primed mice. The B cell hybridoma was generated by the fusion of P3X6 Ag 8.6.5.3 mouse myeloma cells with immunized mouse splenocytes. The ZV-16 antibody specifically recognizes the E glycoprotein, is non-neutralizing, and does not cross-react with Dengue, Japanese encephalitis, or West Nile viruses.

ATCC® VR-1866™ specifically recognizes ZIKV-infected cells in indirect immunofluorescence assays. The clone ZV-16 antibody is also reported to function in ELISA and in western blot assays performed under non-reducing conditions.

Name of Depositor MS Diamond, Washington University School of Medicine
Chain of Custody ATVC <-- MS Diamond, Washington University School of Medicine
Source Mouse monoclonal antibody prepared against the envelope (E) glycoprotein of Zika virus (ZIKV) was purified from ascites fluid by protein G affinity chromatography.
References

Miner JJ, et al. Zika Virus Infection during Pregnancy in Mice Causes Placental Damage and Fetal Demise. Cell 165(5): 1081-1091, 2016. PubMed: 27180225

Zhao H, et al. Structural Basis of Zika Virus-Specific Antibody Protection. Cell 166(4): 1016-1027, 2016. PubMed: 27475895

MS Diamond, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • For-profit customers must obtain a research use license agreement from the Contributor prior to shipment.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Restrictions

This material is subject to the following restrictions in addition to those outlined in the ATCC Material Transfer Agreement:

  1. Prior to purchase, any for-profit Purchaser must obtain a research use license from Washington University; and
  2. When a Commercial Use is contemplated, all for-profit and non-profit Purchasers must obtain a Commercial Use license from the contributor.  For information on executing a license to use this product for research (for-profit entities) or Commercial Use (for-profit and non-profit entities), contact:

Washington University Office of Technology Management
Attn. Associate Director
E-mail: otm@wustl.edu 
Phone: 314.747.0920.