To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at sales@atcc.org. For Technical questions please contact tech@atcc.org. Thank you.
X

Human rhinovirus 16, Purified (ATCC® VR-283PQ)

Classification: Picornaviridae, Enterovirus  /  Product Format: frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL

Permits and Restrictions

View Permits

Classification Picornaviridae, Enterovirus
Deposited As Rhinovirus 16
Agent Human rhinovirus 16, Purified
Strain 11757
Common Name Rhinovirus 16, HRV-16
Applications Respiratory research
In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL
Storage Conditions -70°C or colder
Comments ATCC® VR-283PQ™ is a high-titer, purified, live virus suspended in 0.01% BSA + 25 mM HEPES + 10 mM NaCl + DPBS. This product was prepared from ATCC® VR-283™ via purification through sucrose cushion.

The pH of the medium should be maintained in a range of 7.0-7.3. Rocking of cultures during adsorption may enhance viral replication and CPE. Deposited as Rhinovirus HRV-16. HA is not demonstrated. Not inactivated by ether.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
H1HeLa (ATCC® CRL-1958™)
Growth Conditions

Temperature: 33°C

Recommendations for Infection: Plate cells approximately 24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking continuously on slow speed. End adsorption by adding virus growth medium.

Incubation: 2-3 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer.

Effect on Host
CPE, cell rounding and sloughing
Name of Depositor RM Conant, VV Hamparian, The Ohio State University; ATCC
Special Collection ATCC
Source Throat swab from a healthy 2-year-old female, Washington, DC, 1960
References

Hamparian VV, Conant RM. Rhinoviruses: a numbering system. Nature 213: 761-763, 1967. PubMed: 4291698

Johnson KM, Rosen L. Characteristics of five newly recognized Enteroviruses recovered from the human oropharynx. Am. J. Epidemiol. 77(1): 15-25, 1963.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation