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Human rhinovirus 14, Purified (ATCC® VR-284PQ)

Classification: Picornaviridae, Enterovirus, Rhinovirus B  /  Product Format: frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL

Permits and Restrictions

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Classification Picornaviridae, Enterovirus, Rhinovirus B
Deposited As Rhinovirus type 14
Agent Human rhinovirus 14, Purified
Strain 1059
Applications
Virucide testing
Respiratory research
In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL
Storage Conditions -70°C or colder
Comments ATCC® VR-284PQ™ is a high-titer, purified, live virus suspended in 0.01% BSA + 25 mM HEPES + 10 mM NaCl + DPBS. This product was prepared from ATCC® VR-284™ via purification through sucrose cushion.
Effect on Host
CPE, rounding and sloughing
Recommended Host
H1-HeLa (ATCC® CRL-1958™)
Growth Conditions

Temperature: 33°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium.

Incubation: 1-5 days while rocking continuously

Effect on Host
CPE, rounding and sloughing
Name of Depositor RM Conant, VV Hamparian, The Ohio State University; ATCC
Special Collection ATCC
Source Throat swab from young adult with upper respiratory illness, South Carolina, 1959
References

Johnson KM, Rosen L. Characteristics of five newly recognized Enteroviruses recovered from the human oropharynx. Am. J. Epidemiol. 77(1): 15-25, 1963.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation