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Influenza A virus (H1N1), Purified (ATCC® VR-95PQ)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 CEID50/mL

Permits and Restrictions

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Classification Orthomyxoviridae, Influenzavirus A
Deposited As Influenza A virus
Agent Influenza A virus (H1N1), Purified
Strain A/PR/8/34
Applications Respiratory research
In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 CEID50/mL
Storage Conditions -70°C or colder
Comments ATCC® VR-95PQ™ is a high-titer, purified, live virus suspended in 1X PBS + 0.1% Sodium azide. This product was prepared from ATCC® VR-95™ via purification through sucrose gradient centrifugation.

Third edition of HHS Publication No. (CDC) 93-8395 notes no reports of laboratory-acquired infection with strain A/PR/8/34.
Effect on Host
Hemagglutinates chicken red blood cells and possible death of chicken embryo
Recommended Host
Specific pathogen free chicken embryonated eggs, 10-11 days
Growth Conditions

Temperature: 35°C

Recommendations for Infection: Dilute virus in D-PBS prior to inoculation.

Incubation: 2-3 days in a humidified atmosphere

Effect on Host
Hemagglutinates chicken red blood cells and possible death of chicken embryo
Name of Depositor WA Chappell, M Coleman, Center for Disease Control and Prevention; ATCC
Special Collection NCRR Contract
Source Patient in Puerto Rico, 1934
References

Francis T. Immunological relationships of strains of filterable virus recovered from cases of human influenza. Proc. Soc. Exp. Biol. Med. 32: 1172-1175, 1935.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation