Rickettsia raoultii (ATCC^® VR-1596^™)

Classification: Rickettsiaceae, Rickettsieae / Product Format: frozen

Rickettsia raoultii ATCC^® VR-1596^™

frozen

For-Profit: $1,020.00 Non-Profit: $850.00

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Permits and Restrictions	View Permits
Classification	Rickettsiaceae, Rickettsieae
Agent	Rickettsia raoultii
Strain	DnS14
Applications	Vector borne research
Positive for Mycoplasma	Yes
Biosafety Level	3 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	Isolated from Dermacentor nutalli in Siberia, in 1994
Product Format	frozen
Storage Conditions	Store at -60°C or colder

Comments	This item is a representative of a newly identified rickettsial species (Rickettsia raoultii), a gram-negative, obligately intracellular and non-motile bacterium. It is genetically identical to the type strain for this species (Khabarovsk, isolated in 2005) at all loci sequenced. The preparation available through ATCC tested positive for mycoplasma contamination (M.orale) by PCR. Requests for this agent require special permits and a signed statement assuming all risks and responsibility for laboratory handling. Bacteria visible in cells following staining with Gimenez stain
Effect on Host	CPE in the form of cell rounding and sloughing of infected cells.

Comments

This item is a representative of a newly identified rickettsial species (Rickettsia raoultii), a gram-negative, obligately intracellular and non-motile bacterium.

It is genetically identical to the type strain for this species (Khabarovsk, isolated in 2005) at all loci sequenced. The preparation available through ATCC tested positive for mycoplasma contamination (M.orale) by PCR.

Requests for this agent require special permits and a signed statement assuming all risks and responsibility for laboratory handling.

Bacteria visible in cells following staining with Gimenez stain

Effect on Host

CPE in the form of cell rounding and sloughing of infected cells.

Growth Medium	ATCC^® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM) Fetal Bovine Serum
Recommended Host	Vero cells (ATCC CRL-1587)
Growth Conditions	Recommendation for Infection: 5 days at 32°C and 5% CO₂ in EMEM (ATCC 30-2003 with 2 mM L-glutamine) supplememted with 2% FBS (ATCC 30-2020), following centrifugation at 700 g for one hour at 22°C

Effect on Host	CPE in the form of cell rounding and sloughing of infected cells.

Name of Depositor	Dr. P. -E. Fournier
Special Collection	ATCC
Year of Origin	1994
References	Mediannikov, O., et. al. 2008. Rickettsia raoultii sp. nov., a spotted fever group rickettsia associated with Dermacentor ticks in Europe and Russia. Int. J Syst. Evol. Microbiol. Jul;58 (Pt 7):1635-39. PubMed: 18599708 Marrero, M and Raoult D. Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am J Trop Med Hyg. 1989 Feb;40(2):197-9. PubMed: 2645804 Rydkina, E. et. al., New Rickettsiae in ticks collected in territories of the former soviet union. Emerg. Infect. Dis. 1999. Nov-Dec;5(6):811-4. PubMed: 10603217

Permits	These permits may be required for shipping this product: USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit. Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS

Permits

These permits may be required for shipping this product:

USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Basic Documentation