Aspergillus oryzae var. viridis Murakami, anamorph (ATCC® 22788)

Strain Designations: RIB 128 [CBS 819.72, IFO 30113, JCM 2248, NJK 72]  /  Product Format: frozen

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Deposited As Aspergillus oryzae var. viridis Murakami
Strain Designations RIB 128 [CBS 819.72, IFO 30113, JCM 2248, NJK 72]
Application
Degrades carbamide
Produces N-desferriferrichrome deferriferrichrome
Produces acid carboxypeptidase
Produces acid protease protease (acid)
Produces amylase, alpha 1A; salivary Amylase, salivary, alpha-1A
Produces galactomannan-protein
Produces glucoamylase
Produces kojic acid
Produces protyrosinase
Produces tyrosinase
Produces urease urea amidolyase
production of urease and degradation of carbamide in sake brewing
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain yes (Samson RA, Gams W. Typification of the species of Aspergillus and associated teleomorphs In Samson RA, Gams W. Advances in Penicillium and Aspergillus systematics. New York, Plenum Press pp. 31-54, 1985.), Ref. . Rep. Res. Inst. Brew. 143: 5, 1971. , RefJ. Gen. Appl. Microbiol. 17: 281-309, 1971.
Preceptrol® no
Comments
Rice koji made from this strain turns brown
Taxonomy
Aflatoxin not produced
Medium ATCC® Medium 336: Potato dextrose agar (PDA)
ATCC® Medium 325: Malt extract agar (Blakeslee's formula)
Growth Conditions
Temperature: 24°C to 36°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

AAGGATCATTACCGAGTGTAGGGTTCCTAGCGAGCCCAACCTCCCACCCGTGTTTACTGTACCTTAGTTGCTTCGGCGGGCCCGCCATTCATGGCCGCCGGGGGCTCTCAGCCCCGGGCCCGCGCCCGCCGGAGACACCACGAACTCTGTCTGATCTAGTGAAGTCTGAGTTGATTGTATCGCAATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAGTGTGAATTGCAGAATTCCGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCATCAAGCACGGCTTGTGTGTTGGGTCGTCGTCCCCTCTCCGGGGGGGACGGGCCCCAAAGGCAGCGGCGGCACCGCGTCCGATCCTCGAGCGTATGGGGCTTTGTCACCCGCTCTGTAGGCCCGGCCGGCGCTTGCCGAACGCAAATCAATCTTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAG


D1D2 region of the 28S ribosomal RNA gene

CATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCTCCTTCGGGGTCCGCATTGTAATTTGCAGAGGATGCTTCGGGTGCGGCCCCTGTCTAAGTGCCCTGGAACGGGCCGTCAGAGAGGGTGAGAATCCCGTCTGGGATGGGGTGTCCGCGCCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAAAAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTCGCCTCCAGGGTTCAGCCGGCATTCGTGCCGGTGTACTTCCCTGGGGGCGGGCCAGCGTCGGTTTGGGCGGCCGGTCAAAGGCTCCCGGAATGTAGTGCCCTCCGGGGCACCTTATAGCCGGGAGTGCAATGCGGCCAGCCTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAATGGTCGTAAACGA

Name of Depositor H Murakami
Isolation
Rice koji for making sake, Japan
Cross References

Nucleotide (GenBank) : D37929 protyrosinase gene, melO

References

Ichishima E, et al. Multiple forms of protyrosinase from Aspergillus oryzae and their mode of activation at pH 3.0. Biochim. Biophys. Acta 786: 25-31, 1984. PubMed: 6424711

Samson RA, Gams W. Typification of the species of Aspergillus and associated teleomorphs In Samson RA, Gams W. Advances in Penicillium and Aspergillus systematics. New York, Plenum Press pp. 31-54, 1985.

. . Rep. Res. Inst. Brew. 143: 5, 1971.

J. Gen. Appl. Microbiol. 17: 281-309, 1971.

. . Biotechnol. Tech. 9: 153-156, 1995.

. . Hakkokogaku 59: 491-500, 1981.

Fujita Y, et al. Molecular cloning and nucleotide sequence of the protyrosinase gene, melO, from Aspergillus oryzae and expression of the gene in yeast cells. Biochim. Biophys. Acta 1261: 151-154, 1995. PubMed: 7893753

. . J. Brew. Soc. Jpn. 78: 475-479, 1983.

Oyashiki H, et al. Screening for strains of Aspergillus oryzae that degrade carbamide for use in sake brewing. J. Ferment. Bioeng. 71: 126-127, 1991.

Nakajima T, Ichishima E. Chemical structure of the galactomannan moiety in the cell wall glycoproteins of Aspergillus oryzae. J. Ferment. Bioeng. 78: 472-475, 1994.

Kumeda Y, Asao T. Single-strand conformation polymorphism analysis of PCR-amplified ribosomal DNA internal transcribed spacers to differentiate species of Aspergillus section Flavi. Appl. Environ. Microbiol. 62: 2947-2952, 1996. PubMed: 8702288

type strain

Samson RA, Gams W. Typification of the species of Aspergillus and associated teleomorphs In Samson RA, Gams W. Advances in Penicillium and Aspergillus systematics. New York, Plenum Press pp. 31-54, 1985.

. . Rep. Res. Inst. Brew. 143: 5, 1971.

J. Gen. Appl. Microbiol. 17: 281-309, 1971.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation