Complete Growth Medium
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Dulbecco's modified Eagle's medium with 200 nM dexamethasone, 96%; fetal bovine serum, 4%
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Subculturing
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Medium Renewal: Every 2 to 3 days Remove medium, rinse monolayer with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
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