L.N. 4159 (ATCC® CRL-10998)

Organism: Mus musculus, mouse  /  Tissue: liver  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue liver
Product Format frozen
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Genes Expressed
temperature sensitive T antigen
Cellular Products
temperature sensitive T antigen
The cells contain the HB101 pLux F70 plasmid.
The cells should be grown at 33C.
Complete Growth Medium Dulbecco's modified Eagle's medium with 200 nM dexamethasone, 96%; fetal bovine serum, 4%
Medium Renewal: Every 2 to 3 days
Remove medium, rinse monolayer with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor Pfizer, Inc.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation