Human primary fibroblasts play an important role in maintaining the structural integrity of connective tissue and in the synthesis of extracellular matrix proteins such as collagens, glycosaminoglycans, and glycoproteins. Fibroblasts are morphologically heterogeneous, and their appearance is dependent on in vivo location and activity. After tissue injury, fibroblasts are stimulated to produce wound healing proteins.
Fibroblasts are mediators in inflammation, cancer, angiogenesis, and pathological tissue fibrosis, and they are frequently used in studies related to wound healing, tissue engineering and regeneration applications, and the support in growth and establishment of pluripotent stem (iPS) cells.
ATCC offers a variety of products for fibroblast culture, including normal human dermal fibroblasts (NHDF), media, and growth kits to support either low-serum or serum-free cell growth.
To improve your clinically relevant respiratory research, we offer primary fibroblast primary cells from COPD, asthma, fibrosis, and cystic fibrosis.
Resources for fibroblast research
Primary Human Fibroblast Culture
ATCC Primary Human Fibroblasts can be cultured in complete growth medium with or without serum. The use of Fibroblast Growth Kit–Serum-Free creates a completely defined medium for the serum-free culture of human fibroblasts. The rate of proliferation is equal to or greater than media supplemented using FBS (at concentrations ranging from 2% to 10%) through 10 population doublings. Learn the cell culture protocols, materials needed, preparation, handling of frozen cells, and more.
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Lung and Gingival Fibroblast Cell Models for In Vitro Toxicity Studies
In this study, the results show that the hTERT-immortalized lung fibroblasts and gingival fibroblasts retain the important physiological characteristics of the primary cells from which they were derived and provide a very useful in vitro cell model for toxicity screening.
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