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145-2C11 (ATCC® CRL-1975™)

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)  /  Cell Type: Hybridoma: B lymphocyte  /  Tissue: Spleen, blood  / 

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Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Tissue Spleen, blood
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast-like
Culture Properties Suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications

It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor. The antibody reacts with the murine T cell receptor (CD3 - T3) complex.

The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.

The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
It reacts with all mature T cells and can both activate and inhibit T cell function.
Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb). Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse CD3
Cellular Products
immunoglobulin; monoclonal antibody; against mouse CD3
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Isotype IgG , IgG
Name of Depositor JA Bluestone
Deposited As hamster, Armenian (B cell); mouse (myeloma)
References

Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524

Kayagaki N, et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524

Kayagaki N, et al. Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079