pBS-Cip1 (ATCC® 79926)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pBS-Cip1
GenBank Number

L25610

Species Homo sapiens, human
Depositors SJ Elledge
Vector
Construct size (kb): 5.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: XhoI
Vector end: XhoI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDKN1A
Other gene symbols: CIP1; WAF1*
Gene name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.2
Tissue: B lymphocyte (EBV-transformed)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: L25610
Insert lengths(kb): 2.099999904632568
Tissue: B lymphocyte (EBV transformed)
Gene product: cyclin-dependent kinase inhibitor 1A (p21, Cip1)( melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)) [CIP1]
Insert Size (kb) 2.100
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.2, 0.66, 0.58, 0.50, 0.34, 0.22; EcoRI--3.8, 1.2; KpnI--3.8, 0.92, 0.31; PvuII--2.8, 1.2, 0.92, 0.4; XhoI--3.0, 2.1.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.14, 0.61, 1.25, 1.8; KpnI--0.92; EcoRI--1.0; XhoI--2.0.
Contains the complete coding sequence (nt 79-573 of the sequence record).
References

Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751