pCFM536 plasmid in E. coli (ATCC® 39934)

Applications: Expression vector  /  Depositors: Amgen, Inc., F Lin, Amgen, Inc.

Designations pCFM536 plasmid in E. coli
Permits and Restrictions

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Depositors Amgen, Inc., F Lin, Amgen, Inc.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Host
Distribution host: Escherichia coli JM103/pMW1
Vector Information
Vector information for pCRM536:

Vector Type: plasmid

Vector size (kb): 4.6

Markers: ampR

Promoter: lambda PL

Replicon: R1

Vector information for pMW1 (ATCC 39933):

Vector Type: plasmid

Vector size (kb): 12.0

Markers: kanR, tetR

Replicon: pSH1

Repressor gene: cI857

Applications
Expression vector
Comments
Ampicillin is the selection marker for pCFM526, kanamycin for pMW1.
Temperature sensitive, grow at 28°C.
This is an expression plasmid containing a lambdaPL promoter, a multiple cloning sequence, and a lambda Toop terminator.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 28°C
References

Lin FK. DNA sequences encoding erythropoietin. US Patent 4,703,008 dated Oct 27 1987

Morris CF. DNA plasmids. US Patent 4,710,473 dated Dec 1 1987

Shipped frozen
Shipping Information Frozen glycerol stock of Escherichia coli containing the plasmid.