pSH2-gamma3 (ATCC® 59620)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Lefranc

Permits and Restrictions

View Permits

Designations pSH2-gamma3
Species Homo sapiens, human
Depositors M Lefranc
Construct size (kb): 13.0
DNA: genomic
Insert lengths(kb): 7.300000190734863
Gene product: immunoglobulin gamma 3 (Gm marker) [IGHG3]
Alleles: A1, A2, A3, A4, A5, A6
Insert Size (kb) 7.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Insert contains internal SphI (2), KpnI (1) and SstI (1) sites.
Restriction digests of the clone give the following sizes (kb): HindIII--7.8, 4.85; PstI--4.2, 2.45, 1.8, 1.65, 0.69; EcoRI--13.0; BamHI--13.0, 0.5; SalI--13.0.
This plasmid shows some instability. We recommend growing on LB agar with ampicillin, and picking colonies to select the correct clone.
Contains the complete gene.

Huck S, et al. Sequence of a human immunoglobulin gamma 3 heavy chain constant region gene: comparison with the other human C gamma genes. Nucleic Acids Res. 14: 1779-1789, 1986. PubMed: 3081877

Huck S, et al. A gamma 3 hinge region probe: first specific human immunoglobulin subclass probe. FEBS Lett. 208: 221-230, 1986. PubMed: 2877902

Marie-Paule Lefranc, personal communication