pJ3H (ATCC® 87187)

Applications: encodes an epitope tag for protein isolation or monitoringexpression vector  /  Depositors: J Chernoff

Permits and Restrictions

View Permits

Designations pJ3H
Depositors J Chernoff
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.5000000000000000
Vector: pJ3H (plasmid)
Promoters: Promoter SV40 Early
Construction: pJ3omega
Construct size (kb): 3.5
Features: marker(s): ampR
other: SV40 t IVS
promoter: SV40 Early
replicon: SV40
replicon: pMB1
MCS: XbaI...BglII
terminator: SV40 T polyadenylation
epitope tag: hemagglutinin (HA)
encodes an epitope tag for protein isolation or monitoring
expression vector
Restriction digests of the clone give the following sizes (kb): BamHI--3.5; EcoRI--3.5; PvuI--1.95, 1.5.
Shuttle expression vector encoding an influenza hemagglutinin (HA) epitope tag, recognized by monoclonal antibody 12CA5, to facilitate protein isolation and monitoring.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Sells MA, Chernoff J. Epitope-tag vectors for eukaryotic protein production. Gene 152: 187-189, 1995. PubMed: 7530674

Shipped freeze-dried