pBKV (34-2) plasmid in E. coli (ATCC® 45025)

Organism: BK polyomavirus, BK Virus  /  Clone Type: Clone  /  Depositors: PM Howley

Permits and Restrictions

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Designations pBKV (34-2) plasmid in E. coli
GenBank Definition

BK polyomavirus isolate BKV34-2, complete genome

Human polyomavirus BK nuclear factor

GenBank Number



Species BK polyomavirus, BK Virus
Depositors PM Howley
Vector Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII
Polylinker sites:
Host range: Escherichia coli

Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR

Cross references: DNA Seq. Acc.: J01749
Insert Genome: BK virus
Gene name: genome, full-length
Contains complete coding sequence?: U
Strain: Dunlop
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 5.0
Cross references: DNA Seq. Acc.: KP412983
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): BamHI--5, 4.4; EcoRI--5.3, 4.1.
pBKV (34-2) from P. Howley
Circular BKV was linearized with BamHI before cloning.
Classification Polyomaviridae, Polyomavirus

Henriksen S, et al. Human BK polyomavirus plasmid pBKV (34-2) (Dunlop) contains mutations not found in the originally published sequences. Genome Announc. 3(2): e00046-15, 2015. PubMed: 25814590

Seif I, et al. The Genome of human papovavirus BKV. Cell 18: 963-977, 1979. PubMed: 229976