(ATCC® 37458)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: P Rotwein

Permits and Restrictions

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Species Homo sapiens, human
Depositors P Rotwein
leukocyte, 68-year-old female, Black, with NIDDM
Tissue: leukocyte, 68-year-old female, Black, with NIDDM
DNA: genomic
Digest: Partial
Insert size range: 15 - 20 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 300000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Titer using Escherichia coli C600 (ATCC 23724 or ATCC 47048).
LambdaMG14 has the left arm from lambda1059 and the right arm from Charon 30 (yeast stuffer fragment). This library was amplified once by Rotwein and once by ATCC.

Rotwein P, et al. Genetic analysis of the hypervariable region flanking the human insulin gene. Am. J. Hum. Genet. 39: 291-299, 1986. PubMed: 2876625

Rotwein P, et al. Organization and sequence of the human insulin-like growth factor I gene. Alternative RNA processing produces two insulin-like growth factor I precursor peptides. J. Biol. Chem. 261: 4828-4832, 1986. PubMed: 2937782