Species
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Saccharomyces cerevisiae Meyen ex E.C. Hansen
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Vector
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Construct size (kb): 42.40000152587891 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHC79 Intact vector size: 6.430 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI Polylinker sites: Construction: pBR322, Charon 4A Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR replicon: pMB1 terminator: none Cross references: DNA Seq. Acc.: L08873
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Insert
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DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae Gene name: DNA Segment Contains complete coding sequence?: U Chromosome: IX; Localization: IX R Strain: AB972 ( ATCC 76269) Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 36.0 Cross references: Insert lengths(kb): 36.0 Gene product: DNA Segment
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Biosafety Level
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1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
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Shipping Information
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Distributed: freeze-dried
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Comments
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Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 3.407, 5.813, 2.064, 3.028, 2.505, 3.971, 0.941, 1.169, 1.031, 1.203, 0.520, 2.072, 1.938, 3.727, 0.899, 0.564, 1.207, 0.473. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.40, 0.49, 0.55, 0.93, 0.96, 1.1, 1.2, 2.0, 2.2, 2.6, 3.3, 3.6, 3.9, 4.2, 6.3. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70193, 70882, 70883, 70895, 71124.
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Classification
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Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
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References
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Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151
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