Nucleotide (GenBank) :
Expression cloning vector pACYC177/ET3d/yNMT, complete sequence.
Distribution host: Escherichia coli BL21(DE3)
Size (kb): 4.9429998397827150
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pACYC177/ET3d
Intact vector size:
Type of vector: plasmid
Vector end: NcoI
Vector end: BamHI
Cloning sites: NcoI, NcoI+BamHI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): kanR, ->
ribosome-binding site: gene 10
promoter: T7 (phi10), ->
Vector: pACYC177/ET3d/yNMT (plasmid)
Promoters: Promoter for expression T7 (phi10)
Construct size (kb): 4.900000095367432
Features: marker(s): kanR
promoter for expression: T7 (phi10)
coding sequence: NMT1
produces protein peptide N-myristoyl transferase glycylpeptide N-tetradecanoyltransferase
Restriction digests of the clone give the following sizes (kb): NdeI--5.5; NcoI--5.5; StuI--3.9, 1.5.
Expression system compatible with ColE1 plasmids, for cotransformation and production of myristoylated proteins.
Introduction of the NcoI site during amplification introduced a single base substitution T->G at nt 4.
Expression of this gene in the presence of 100 uM myristic acid allows myristoylation of recombinant proteins produced by a second ColE1 plasmid.