Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47076)

Strain Designations: MG1655  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations MG1655
Application Transformation host
Bacteriophage host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Shipping Information Frozen gycerol stock of E. coli
Storage Conditions -80°C or colder
Preceptrol® no
Genotype F- lambda-
Type Strain no
Electronic access to the correlations between the restriction map and the ordered phage library of Kohara are available over the Internet by anonymous ftp at these hosts: and
Host strain with well-characterized restriction maps for NotI, SfiI, XbaI and BlnI. Can be used as a molecular size marker for pulsed-field gel electrophoresis.
AE000111 through AE000510 are individual GenBank numbers for the complete sequence, U00096
Derived from the parent strain W1485 by curing of the F plasmid.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype F- lambda-
Name of Depositor GM Weinstock, University of Texas Medical School
Chain of Custody
ATCC <-- GM Weinstock <-- MG1655

Blattner FR. Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 21: 5408-5417, 1993. PubMed: 8265357

Blattner FR, et al. The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1462, 1997. PubMed: 9278503

Burland V, et al. DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication. Genomics 16: 551-561, 1993. PubMed: 7686882

Daniels DL, et al. Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes. Science 257: 771-778, 1992. PubMed: 1379743

Dicker IB. Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli. J. Bacteriol. 173: 334-344, 1991. PubMed: 1987124

Heath JD, et al. NotI genomic cleavage map of Escherichia coli K-12 strain MG1655. J. Bacteriol. 174: 558-567, 1992. PubMed: 1729244

Perkins JD, et al. XbaI and BlnI genomic cleavage maps of Escherichia coli K-12 strain MG1655 and comparative analysis of other strains. J. Mol. Biol. 232: 419-445, 1993. PubMed: 8393935

Plunkett G III, et al. Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 21: 3391-3398, 1993. PubMed: 8346018

Johanson U, Hughes D. Comparison of the complete sequence of the str operon in Salmonella typhimurium and Escherichia coli. Gene 120: 93-98, 1992. PubMed: 1398129

Jensen KF. The Escherichia coli K-12 'wild types' W3110 and MG1655 have an rphframeshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. Gene 175: 3401-3407, 1993. PubMed: 8501045

Freddolino PL, Amini S, and S Tavazoie. Newly Identified Genetic Variations in Common Escherichia coli MG1655 Stock Cultures. J. Bacteriol. 194(2): 303-306, 2012. PubMed: 22081388

Cross References

Nucleotide (GenBank) : U00096 complete sequence

Nucleotide (GenBank) : X72920 intercistronic region

Nucleotide (GenBank) : X64592 gene for ribosomal protein S7, partial

Nucleotide (GenBank) : L10328 chromosomal region from 81.5 to 84.5 minutes

Nucleotide (GenBank) : L19201 chromosomal region from 87.2 to 89.2 minutes

Nucleotide (GenBank) : M87049 chromosomal region from 84.5 to 86.5 minutes

Nucleotide (GenBank) : U00006 chromosomal region from 89.2 to 92.8 minutes

Nucleotide (GenBank) : X54797 firA gene, firA(200) and firA(201) allele, and skp gene (3' end)

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation