S. cerevisiae/E. coli pRS400 shuttle vectors (ATCC® 87538)

Depositors: P Hieter, JD Boeke, TW Christianson

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Designations S. cerevisiae/E. coli pRS400 shuttle vectors
Depositors P Hieter, JD Boeke, TW Christianson
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli DH5alpha
Vector Information
Intact vector size: 4.898
Type of vector: phagemid
Cloning sites: KpnI ApaI XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI EagI
NotI SacII SacI NaeI
Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI
SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII
Construction: pBluescriptII KS+
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 417-1219
replicon: f1, <-, 1351-1807
insert detection: lacZ', <-, 1800-2137
promoter for in vitro transcription: T7, ->, 1977-1996
MCS: KpnI...SacI, ->, 2004-2111
promoter for in vitro transcription: T3, <-, 2123-2142
promoter: lac, <-, 2212-2240
replicon: pMB1, 2566-2566
marker(s): ampR, <-, 3324-4184
centromere: CEN6, 4326-4442
replicon: ARSH4, 4443-4806
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

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