pSV2-dhfr (ATCC® 37146)

Applications: expression vectorshuttle vector  /  Depositors: P Berg

Designations pSV2-dhfr
Permits and Restrictions

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Depositors P Berg
Biosafety Level 1
Vector Information
Size (kb): 4.90
Vector: pSV2-dhfr (plasmid)
Promoters: Promoter SV40
Construction: pBR322, SV40, mouse Dhfr
Construct size (kb): 4.90
Features: marker(s): ampR, mtxR
promoter: SV40
replicon: pMB1
expression vector
shuttle vector
Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--5.2; PvuII/HindIII--4.8, 0.3; BglII/EcoRI--3.6, 1.5; AccI/HindIII--3.6, 0.54, 0.44, 0.27; AccI--3.6, 0.96, 0.27; HindIII--5.0; PstI--4.1, 0.9.
Shuttle vector conferring selective advantage to mammalian cell lines grown in the presence of methotrexate. Permits selection in dhfr- cell lines.
Inserts cloned into the PvuII site next to the SV40 late promoter, may be expressed in mammalian cells.
This vector also contains a copy of the mouse dyhydrofolate reductase (dhfr) gene under control of the SV40 early promoter. Dhfr confers methotrexate resistance in Chinese Hamster ovary cells (CHO) which lack dihydrofolate reductase.
This vector does not contain the Large T antigen from SV40.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Subramani S, et al. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1: 854-864, 1981. PubMed: 9279398

Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of E. coli containing the plasmid.