Rickettsia rhipicephali (ATCC® VR-1638™) Classification: Rickettsiaceae, Rickettsieae / Product Format: frozen 1 mL per vial General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Classification Rickettsiaceae, Rickettsieae Agent Rickettsia rhipicephali Strain CWPP Biosafety Level 3 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Product Format frozen 1 mL per vial Storage Conditions -70°C or colder Effect on Host CPE, rounding and sloughing Recommended Host Vero (ATCC® CCL-81™) Growth Conditions Temperature: 34°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of agent (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb by centrifugation at 800 x g for 30 minutes. End adsorption by adding agent growth medium. Incubation: 6-20 days Effect on Host CPE, rounding and sloughing Name of Depositor GA Dasch, Naval Medical Research Center Permits These permits may be required for shipping this product: USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit. Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS