Chlamydia muridarum Everett et al. (ATCC® VR-123)

Classification: Chlamydiaceae  /  Product Format: frozen

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Classification Chlamydiaceae
Deposited As Chlamydia trachomatis (Busacca) Rake
Agent Chlamydia muridarum Everett et al.
Strain Mouse Pneumonitis strain Nigg II
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Although this agent is not known to be pathogenic for man, it should be handled with care. Mice receiving 1:100 dilution i.n. of infected y.s. occasionally develop pneumonia and die.
The Nigg II strain was first deposited by F. B. Gordon as yolk-sac adapted material. It was subsequently redeposited as tissue culture adapted material by J. Schachter
This preparation has tested positive for Mycoplasma contamination
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Recommended Host
McCoy (ATCC® CRL-1696™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: 48 hours at 37°C in McCoy cells (ATCC® CRL-1696™). Add glassbeads and vortex preparation to disrupt cells.  Infect monolayer with disrupted material.  Centrifuge at 3000 x rpm (750 x g) for 1 hour.  Feed with fresh growth medium containing 1-2 µg/mL cycloheximide.  Incubate at 37°C for 48 hours.
Incubation: 48 hours
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Name of Depositor FB Gordon, J Schachter
Source Normal mice, 1939
Year of Origin 1939

Pal S, et al. Immunization with an acellular vaccine consisting of the outer membrane complex of Chlamydia trachomatis induces protection against a genital challenge. Infect. Immun. 65: 3361-3369, 1997. PubMed: 9234798

Nigg C. An unidentified virus which produces pneumonia and systemic infection in mice. Science 95: 49, 1942.

Pal S, et al. Intranasal immunization induces long-term protection in mice against a Chlamydia trachomatis genital challenge. Infect. Immun. 64: 5341-5348, 1996. PubMed: 8945586

Kaushic C, et al. Chlamydia trachomatis infection in the female reproductive tract of the rat: influence of progesterone on infectivity and immune response. Infect. Immun. 66: 893-898, 1998. PubMed: 9488372

Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462

type strain

Cross References

Nucleotide (GenBank) : AJ012177 Chlamydia muridarum partial rnpB gene for RNase P RNA subunit.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation