Rickettsia slovaca (ATCC® VR-1639)

Classification: Rickettsiaceae, Rickettsieae  /  Product Format: frozen 1 mL per vial

Classification Rickettsiaceae, Rickettsieae
Agent Rickettsia slovaca
Strain B
Biosafety Level 3

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host CPE, rounding
Recommended Host Vero (ATCC® CCL-81™)
Growth Conditions Temperature: 32°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of agent (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb by centrifugation at 800 x g for 30 minutes. End adsorption by adding virus growth medium.
Incubation: 14 days
Effect on Host CPE, rounding
Name of Depositor GA Dasch, Naval Medical Research Center