Chlamydia trachomatis Serovar Ba (ATCC® VR-347)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen 1 mL per vial

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Classification Chlamydiaceae, Chlamydia
Deposited As Trachoma serotype ba
Agent Chlamydia trachomatis Serovar Ba
Strain Apache-2
Sexually Transmitted Disease Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images Chlamydia trachomatis ATCC VR-347 Images
Comments FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

Please see ATCC Virology Guide for more detailed information on propagation.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining
Recommended Host
McCoy cells (ATCC CRL-1696™)
Growth Conditions
Temperature: 36°C
Recommendations for Infection: For best results, infection should be performed on a 80-100% confluent, 24-48 hour old cellular monolayer. Sonicate seed material for 20 seconds at approximately 240W to disrupt cells.  Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2).  Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour.  End adsorption by adding agent growth medium.
Incubation: Incubate infected culture for 72 hours at 36°C in a humidified 5% CO2 atmosphere.
Preservation: Controlled rate freeze.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining
Name of Depositor E Jawetz
Special Collection ATCC
Source Eye of an Apache Indian child from San Carlos, Arizona

Hanna L, et al. Trachoma viruses isolated in United States. I. Growth in embryonated eggs. Proc. Soc. Exp. Biol. Med. 104: 142-145, 1960. PubMed: 14399529

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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