Influenza A virus (H1N1) (ATCC® VR-1738)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen

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Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H1N1)
Strain A/Virginia/ATCC3/2009
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

This item was isolated in tissue culture.
Product Format frozen
Storage Conditions  -70°C or colder
Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin.
Effect on Host
CPE, rounded refractile cells with cell degeneration
Recommended Host
Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plate cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI 0.1. Adsorb 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Grow in serum free media that contains 2.0μg/ml TPCK-treated trypsin.
Incubation: 2-5 days
Effect on Host
CPE, rounded refractile cells with cell degeneration
Name of Depositor ATCC
Special Collection ATCC
Nasopharyngeal specimen from a patient positive for Flu A in Virginia, 2009.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation